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(2013) Effect of post-menopausal status and age at menopause on type 2 diabetes and pre-diabetes in Japanese individuals: Toranomon hospital health management centre study 17 (TOPICS 17)

ScienceDirect , doi:10.1016/j.freeradbiomed.2017.12.007

Funding This work was supported by the National Key R&D Program (2016YFD0200305), the Young Elite Scientists Sponsorship Program by CAST (2018QNRC001), the Innovative Research Team Development Plan of the Ministry of Education of China (IRT_17R56) and the Fundamental Research Funds for the Central Universities (KYT201802)

Intracellular ferrous iron (Fe) was measured with an enzyme-linked immunosorbent assay (ELISA)-based kit (Wuhan Elabscience Biotechnology Co., Ltd., China), following cell collection, washing, lysis, and processing as per the manufacturers instructions, with absorbance read at 450 nm