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O.et al

New analytical techniques and current advancements in mass spectrometry allow for comprehensive data collection and a better understanding of the effects of different raw materials, starter cultures, fermentation temperature, and storage on the metabolite profile

R&D Systems, Minneapolis, MN) in RPMI-1640 (Gibco, Detroit, MI) medium supplemented with 2% penicillin/streptomycin (Gibco), 20 mM HEPES (Gibco) and 5% pooled normal human heat-inactivated and sterile-filtered AB serum (Innovative Research, Novi, MI) for 7 days, with a media change on day 5 with fresh M-CSF (10 ng/mL)