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doi: 10.3390/jcm12175504 10 CaudriDNixonGMNielsenAMaiLHafekostCRKapurNet al

As shown in Figure 7, unfolded protein with a His tag, e.g., solubilized inclusion bodies by GdnHCl, can easily bind to IMAC (here immobilized Ni ions) resin and be eluted by imidazole