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Peptides were then on-line eluted into a Fusion Tribrid mass spectrometer (Thermo Scientific, San Jose, CA) from an EASY PepMap TM RSLC C18 column (2m, 100, 75m x 50cm, Thermo Scientific, San Jose, CA), using a hold of 6% solvent B (80/20 acetonitrile/water, 0.1% formic acid) for 15 minutes, followed by a gradient of 6-28% solvent B in 105 minutes, then 28-49% solvent B in 15 minutes, 40-100% solvent B in 10 minutes, a 10 minute hold of 100% solvent B, a return to 5% solvent B in 3 minutes, and finally a 3 minute hold of 5% solvent B

Full details are provided in the Supplemental Methods

P., Delous, G., Zalko, D., Vigui, C

10.1073/pnas.1410104111 Proc

However, the individual ingredients in these injections have solid research behind them