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At the molecular level, 5-amino-1MQ functions as a competitive inhibitor of NNMT, demonstrating remarkable potency with an IC of 1.2 0.1 M under standard assay conditions (50 M SAM, 100 M nicotinic acid). This represents a dramatic 10-fold improvement over the parent compound 1-methylquinolinium, achieved through strategic amino group substitution that enhances binding affinity to the NNMT active site. The compound's mechanism centers on preventing the methylation of nicotinamide to 1-methylnicotinamide (1-MNA), thereby preserving nicotinamide for recycling back to NAD+ through the salvage pathway. This intervention effectively blocks what researchers have termed the "NNMT metabolic drain" a process that simultaneously depletes NAD+ precursors and consumes cellular methylation capacity

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mansoni GST (Figure 1C)

Both of these NPs can eliminate ROS, which is known to play a significant role in causing neuronal damage through neuroinflammation (106)

Haem-derived iron is essential for mitochondrial respiration Although endosomal uptake is well established in intracellular iron dynamics 20,21 , the mechanism behind haem-derived iron via HMOX remains largely unknown