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Description

A.SulserF

Briefly, COS cells were transfected with an expression plasmid encoding 3MST- or rhodanese-cDNA using TransIT-LT1 Transfection Reagent (Mirus Bio, Madison, WI, USA) following the procedure recommended by the manufacturer

When the oxidative stress state persists and the damage progresses, senescent cells enter a senescent state marked by p16 upregulation, which consequently inhibits the kinases CDK4 and CDK6, thereby maintaining the stagnant state of the cell cycle [133, 134]