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Acknowledgements The authors would like to thank the experimental apparatus and technical support provided by the Key Laboratory for Metabolic Diseases in Chinese Medicine, Nanjing University of Chinese Medicine

All authors approved the manuscript for publication

Acetyl-L-Carnitine: Benefits and Side Effects Genes Mentioned Contents Acetyl-L-Carnitine (ALCAR) is the acetylated form of the amino acid L-carnitine (LC), which means ALCAR is L-carnitine tagged with an acetyl molecule

However, during high-fat diet (HFD) feeding, obesity and insulin resistance, the accumulation of medium-chain and long-chain acylcarnitines due to incomplete -oxidation may negatively regulate the expression of nuclear-encoded mitochondrial genes, further reducing mitochondrial number and function and creating a vicious metabolic cycle [33]