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For analyzing the spontaneous activity, spontaneous events were identified as peaks in fluorescence above an empirically determined threshold

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doi:10.1002/ptr.3502 Rodrguez-Ramiro I, Ramos S, Bravo L, Goya L, Martn M (2011) Procyanidin B2 and a cocoa polyphenolic extract inhibit acrylamide-induced apoptosis in human Caco-2 cells by preventing oxidative stress and activation of JNK pathway