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Analysis of ileal contents is a more reliable method for assessing amino acid digestibility in poultry as it takes into account the hindgut fermentation, preventing underestimation of the amino acid requirement (Ravindran et al., 2005)

It can also cross into breast milk

Therefore, this limitation should be oriented to interpret the effectiveness of ALC for HE

The reasons for the failure of B12 levels to improve after settlement are likely to be complex and multifactorial