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Numerous key parameters affect the efficacy of MSCs, covering aspects such as the type of transplanted cells, cell source, culture and expansion conditions, transplantation route, cell number, patient selection, and efficacy evaluation indicators

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One-carbon metabolism requires B vitamins, and hence the efficient dietary supply may protect against cancer by reducing DNA instability and by affecting DNA methylation [42], but vitamin B12, methylcobalamin, and coenzyme B12 were found to enhance DNA methylation in the presence of S-adenosylmethionine for concentrations up to 1 M, but at higher concentrations, these compounds were found to inhibit DNA methylation [43]