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After being placed for 1h, the filtrate was filtered, and then the pH was then adjusted to 1.52 at 40 C, kept at 80 C for 30 min, and allowed to stand for 1216 h

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Cys-FeS clusters in disposable cuvettes sealed with parafilm were stable for 3 h, as indicated by the deterioration of the 420 nm signal during UV-Vis analysis (Fig

Subsequently, the mixture was boiled for 15 min, followed by a cooling process and subsequent centrifugation at 10,000 revolutions per minute for 5 min

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