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For LC-MS analysis, samples were thawed on ice-water bath, then 40 L of whole blood was injected into 450 L cold extraction solvent comprising of mixture of Acetonitrile:Methanol:Milli-Q water in ratio 40:40:20 according to protocol described in Presterud et al 35

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( Comparable findings have been reported in models of cerebral ischemia and skeletal muscle injury, where administration of NAC 30 min prior to HBOT suppressed ROS-dependent signaling pathways, including HIF-1 activation, angiogenesis, and tissue regeneration ( In vitro studies further support this concept

J.324(1 Jun Pt