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In June 2022, the MHRA published advice, following a review in Europe, which concluded that this side effect occurs more frequently than was previously thought (MHRA, 2022)

For microinjections into pronuclei of fertilized eggs derived from the superovulated B6C3F1 mouse strain (Charles River, Japan), DNA was diluted to ~2 ng/l by the injection buffer and tails of transgenic founder (F0) pups or yolk sacs of F0 embryos were collected for the genotyping

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