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Dialysis procedures can result in the loss of amino acids, ranging from 4 to 13 g

The following day, membranes were washed with fresh 1 PBS with 0.1% Igepal and incubated for 45 min with gaot anti-mouse (ab6789, Abcam, 1:3,000) conjugated to horseradish peroxidase at a 1:3,000 dilution, and analyzed by enhanced chemiluminescence (ECL, Cytiva) using a Vilbert-Lourmat Photodocumentation Chemistart 5000 imager

Graziani V, Rodriguez-Hernandez I, Maiques O, Sanz-Moreno V

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promote the proliferation of granulosa cells by reducing the apoptosis process leading to cell death support the oocyte maturation that was impaired in this mice model promote the synthesis of sex steroid hormones Other in-vivo studies have demonstrated the benefits of curcumin in supporting follicle development and steroidogenesis (64-66)