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For cell viability assays, medium was supplemented with 10% dialyzed FBS

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Pathological changes in astrocytes in various models of PTSD are similar to those in models of depression

Tang J, Long W, Yan L, Zhang Y, Xie J, Lu G, et al

In contrast, the following features indicate pain sources outside the bladder and a higher risk of failure: Large capacity under anesthesia Absence of Hunner lesions, Lack of relief with local anesthetic bladder instillations, Pelvic muscle tightness/tenderness, Genital hyperesthesia, Pain beyond the pelvis, such as the presence of widespread pain, and, most important, Polysymptomatic or polysyndromic presentation