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10.1021/acs.jafc.3c06015 44 LiangZ.ChenY.XuL.QinM.YiT.ChenH.et al (2015)

However, synthetic versions do exist and are produced by recombinant DNA technology

Ice-crystal formation during each freeze-thaw cycle inflicts mechanical damage on molecular structure, and pre-splitting eliminates the cumulative loss that comes from thawing one vial multiple times