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& Nozik-Grayck, E

In particular, the inhibition of eNOS results from the PKC phosphorylation of Thr495, amplified by the simultaneous dephosphorylation of Ser-1177 (22)

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Omission of the reducing step, which would prevent the release of glutathione residues and the generation of free sulfhydryl groups, resulted in absence of fluorescence ( Figure 2B ), supporting efficiency of the blocking step