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10.1016/j.trim.2007.06.002 270 TanakaH.ItoY.ShimazakiH.TsurumaK.ShimazawaM.HaraH

After stimulation, the cells were rinsed once with 2 ml of KREBs Ringer solution and incubated for 5 min with 5 M CM-H 2 DCFDA

In fact, OS is among the main hypothesized risk factors for male reproductive issues: while capacitation and acrosome reaction depend on physiologic OS, an imbalance between ROS production and impaired clearance can lead to OS within sperm [204]