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Three different types of ERS sensors have been identified

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In particular, 5-Methyl-THF, the main form of folate in the plasma, in order to be absorbed by the cells exploits folate receptor (FR-), mostly expressed in the proximal tubules of kidneys, the choroid plexus, and the placenta ( Two other receptors are known, namely and , that show lower affinity for 5-Methyl-THF in respect to FR-

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The cells were trypsinized and lysed with the lysis buffer provided in the commercial kit on ice

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