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Isoelectric focusing (IEF) was carried out with commercially available immobilized pH gradients (pH 311 nonlinear, 35.6 nonlinear, 47 linear, 69 linear, 24 cm), using the IPG Phor (Amersham Biotech, Amersham, UK) apparatus

For this reason, antihistamines are mainly used in acute or severe cases, or when it is not possible to follow a low-histamine diet [8], or when the administration of drugs that may release histamine is unavoidable [93]

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Application of this assay to analyze plasma from patients with known oxidative stress (sickle cell disease and sepsis) and from a patient with sickle cell disease treated with the antioxidant N-acetylcysteine suggests that cysteine disulfides, in particular protein-bound cysteine, serve as sensitive plasma biomarkers for the extent of oxidative stress and effectiveness of antioxidant treatment

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