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SKU: 9397797900
Price: 25.96 USD
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Description

10.1186/s10194-018-0956-x J

Briefly, protein sample extracts were denatured and separated on 10% polyacrylamide, 0.1% SDS (SDS-PAGE), and transferred onto a nitrocellulose blotting membrane (GE Healthcare, Milan, Italy)

At each time point, 1/10 volume of culture medium per well CCK-8 solution reagent was added, incubated at 37C for 3 h, and the absorbance was measured at 450 nm using a microplate reader