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Always use aseptic techniques during handling to prevent issues like contamination or aggregation, which could compromise the research

The vessels of these areas were counted at high power field (400) by Image Pro- plus 6.0 software and the average microvessel number expressing human CD31 and mouse CD31 in the five fields of interest was scored

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Acidic solutions (pH 4-6) are often preferred to reduce the risk of peptide aggregation and deamidation

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