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First, Atto-647N labeled endogenous proteins were excited with the 647 nm laser (515% maximal laser power), and second, Alexa-594 labeled endogenous proteins were excited using the 594 nm laser (512% maximal power)

The reconstituted solution requires refrigeration and careful handling to maintain potency

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Data was plotted with ggplot2 and Pairwise comparisons were done using a MannWhitney U-test corrected for multiple comparisons using Benjamini and Hochberg as implemented in R (4.2.3)