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The lysates (2 g of protein per lane) in RIPA buffer (Sigma-Aldrich, Cat# R0278) containing a protease inhibitor cocktail as well as phosphatase inhibitor cocktails 2 and 3 (Sigma-Aldrich) were subjected to SDS-PAGE on 520% gradient gels (SuperSep Ace, FUJIFILM-Wako) followed by immunoblotting

This was observed in 2 different immortalized cell lines (U251, U118)

In another set of experiments, animals (n = 15 for each group) were randomly divided as described above and monitored for assessing mortality for 5 days after a lethal dose of APAP (600 mg/kg) or saline administration

Effect of probiotic supplementation on seizure activity and cognitive performance in PTZ-induced chemical kindling