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Lipids were separated by gradient elution with solvent A (acetonitrile/water, 1:1, v/v) and B (isopropanol/acetonitrile/water, 85:15:5, v/v) both containing 5 mM NH 4 HCO 2 and 0.1% (v/v) formic acid

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Treatment starts with injections three times per week for three weeks, then continues weekly

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