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Clinics that want to stay clear of warning letters should audit their websites, intake forms, and social channels now for any language that implies these peptides are FDA-approved or that PCAC review has happened

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The following ligands and inhibitors were used: Recombinant human TGFb1 protein (R&D Systems), recombinant human TGFb2 protein (R&D Systems), recombinant human TGFb3 protein (R&D Systems), tideglusib (Sigma-Aldrich), GSK3B-inhibitor VIII (Sigma-Aldrich), or SM16 (Tocris, Minneapolis, MN, USA) Lactate dehydrogenase cytotoxicity assay Lactate dehydrogenase (LDH), as a measure for cytotoxicity, was quantified by spectrophotometric measurement of the absorbance at 340 nm, which is an indirect measurement of the consumption of nicotinamide adenine dinucleotide (NADH) by LDH

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By inhibiting NF-B, DSF can prevent the upregulation of TGF- and other fibrotic mediators