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In this assay, GPX reduces hydroperoxides, producing oxidized glutathione (GSSG), which is then recycled back to its reduced state by glutathione reductase (GR) and NADPH

MTX-induced apoptosis was significantly attenuated with all treatments except for NTZ-100, where, as depicted in Figures 5AD, Bcl-2 level was augmented while Bax was reduced, and consequently, Bax/Bcl-2 ratio was also reduced as well as caspase-3 activity all when compared to MTX group

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The beneficial effects of zinc on lipid metabolism extend to improved cardiovascular outcomes