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The standard procedure and reagents from the Q5 Site-Directed Mutagenesis kit (NEB) were used to insert mutations, ligate plasmids and transform them into NEB 5 competent cells by heat shock

The findings of these murine studies are in accord with the findings of our preliminary studies conducted in healthy subjects 15

These scaffold-based approaches highlight an important principle: GHK-Cu may be most effective for bone regeneration when delivered locally and in a sustained manner, rather than through systemic injection alone