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Metalloproteinase regulation studies document MMP1 and MMP2 gene expression increases at 0.01 nM with TIMP1 increases at all tested concentrations (0.01-100 nM)

HEK293 Human embryonic kidney cells 293 (HEK293), Madin Darby canine kidney cells (MDCK), and B16F10 murine melanoma cells were grown in DMEM, 10% fetal bovine serum (FBS)

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