Vitamin B12 is one of those nutrients that you dont think much about until you start to feel the effects of not having enough
The experimental trail included a 26-week treatment period, a dose-escalation period of up to 6 weeks, and a 6-week follow-up period after the end of treatment

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Gel lanes were cut in several pieces, and proteins were reduced, alkylated, and digested by overnight incubation with trypsin (bovine sequencing grade, Roche Applied Science, Monza, IT) according to published protocols ( 2.10 Identification of selected antigen by LC-MALDI Protein immunopurifications were precipitated in ice-cold acetone overnight and resuspended in 40 L of NH 4 HCO 3 50 mM, urea 2 M solution, and digested with 5 L of trypsin solution (0.2 g/L) overnight at 37C