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HPLC or UHPLC Chromatography can separate detectable components and report the relative area associated with a principal peak

While the RDA is 2.6 mcg, studies show that higher doses of up to 1,000 mcg (1 mg) daily are generally considered safe during pregnancy, especially when treating deficiency [*]

(1998) Effects of low-dose recombinant human insulin-like growth factor-I on insulin sensitivity, growth hormone and glucagon levels in young adults with insulin-dependent diabetes mellitus

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