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0.5 mL of supernatant was added to 1 ml of buffer and 0.1 mL of 1 mM/L DTNB, mixed well, and left for 10 min before reading at 405 nm

Furthermore, Trx/GSH system inhibitors have demonstrated synergistic antitumor effects in cancer therapy (185)

IN DFO in rats resulted in drug concentration in the cortex approximately 10-fold higher than an equivalent intravenous dose, with lower systemic concentrations and systemic side effects (Hanson et al., 2009)

FSP1 acts as a NAD(P)H-dependent ubiquinone (CoQ) oxidoreductase to suppress lipid peroxidation and ferroptosis via reducing CoQ to ubiquinol (CoQH 2 ) [67, 69]