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[9, 11] Inadequate dietary intake, especially in vegetarians and vegans, leads to a deficiency in vitamin B12

However, because of the processed foods in our modern diets, experts believe the levels of intrinsic factor have been decreasing

Gliadin, zonulin and gut permeability: effects on celiac and non-celiac intestinal mucosa and intestinal cell lines

If your protocol requires a 500 mcg dose, the calculation looks like this: Volume to Draw = Desired Dose (mcg) / Concentration (mcg/mL) Volume to Draw = 500 mcg / 2000 mcg/mL = 0.25 mL So, you would carefully draw 0.25 mL of your solution to get a perfect 500 mcg dose