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From this series of studies, it can be concluded that the addition of OH Py 2 N 2 to LECs activates the translocation of Nrf2 and the expression of antioxidant enzymes, including Grx1 and Grx2 as a mechanism that results in the catalytic protection of LECs against ROS assaults

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Primary antibody solutions were prepared in UltraCruz TM blocking reagent using the following dilution factors: 1:200 rabbit anti-SERF2 (Proteintech, 11691-1-AP), 1:1000 mouse anti-G3BP1 (BD Biosciences, 611127), 1:1000 mouse anti-Fibrillarin (Boster Bio, M03178-3), 1:100 mouse anti-Nopp140 (Santa Cruz, sc-374033), 1:100 mouse anti-USP10 (Santa Cruz, sc-365828), 1:200 mouse anti-FUS (Thermo Fisher, 50-554-337), 1:500 mouse anti-BG4 (Absolute antibody, Ab00174-1.1), 1:100 mouse anti-TIA1 (Santa Cruz, sc-398372), and 1:100 mouse anti-eIF2 (Santa Cruz, sc-133132)