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After isolation, the nuclei were analyzed using the Nexcelom Cellometer Auto2000 with the AOPI fluorescent staining method, followed by tagging of open chromatin regions with Tn5 transposase

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This receptor selectivity makes it an ideal clean GHRH-R agonist for studies where the specific contribution of the GHRH pathway needs to be isolated from ghrelin/GHS-R1a biology

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