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In order to control for a participants initial anxiety that may elevate cardiovascular measures, heart rate and blood pressure were assessed twice prior to gum administration, with only the second assessment included in analyses, as well as another assessment 30 minutes following administration of nicotine or placebo

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Using liquid chromatography-mass spectrometry (LC-MS), they detected compounds of betel nut in the molars of one individual, a young woman known as Burial 11. The chemicalsarecoline and arecaidineare major psychoactive compounds found in the areca nut, known as betel nut

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