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Although these marketing tactics are banned for cigarette and smokeless tobacco companies by the 1998 Master Settlement Agreement and the 2009 Family Smoking Prevention and Tobacco Control Act (put into effect due to the close association with sponsoring music, sports, and other cultural events with youth tobacco use), e-cigarette companies have been able to skirt these restrictions by claiming that their products are different than conventional cigarettes

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After transfection, 1 mL of 10% FBS human macrophage cell culture medium was added to each cuvette

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