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As our in vivo scRNA-seq data did not wholly capture the heterogeneity reflective of this progression, we studied the process by culturing basal tracheal epithelial cells at airliquid interface (ALI) and harvesting replicate cultures at 20 timepoints across mucociliary differentiation for scRNA-seq analysis (Fig

The justification the tobacco industry has used against tax raises, and has often been successful in persuading the government, is that it will increase the number of cigarettes smuggled into the market and this will reduce government tax income