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Subsequently, after treatment, cells were fixed with 2% glutaraldehyde (#G5882, Merck, Darmstadt, Germany) in 0.1 M cacodylate buffer for 1.5 h

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Sometimes (usually in the case of super light or ultra light cigarettes), smokers instinctively cover the perforations on the filters with their lips or fingers as they draw in, resulting in a very high intake of nicotine and tar from the cigarette (1)

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