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Change in plasma nicotine levels from baseline to 65 minutes was the independent variable and age, gender, smoking duration and consumption, FTCD and puff duration were introduced to the analysis as covariates

The combination of high nicotine concentrations delivered efficiently to a physiologically vulnerable adolescent population raises significant concern for both acute events (in susceptible individuals) and the long term development of cardiovascular disease (10, 1214)

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However, a research constraint with assessing the impact of e-cigarettes is that most vapers were formerly smokers, or are dual-users of ENDS and tobacco, making it difficult to isolate the impact of ENDS

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