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The plantlets of 60 days were transplanted into a tray with a nutrient solution for 3 days in growth chamber, and then were exposed to the abiotic treatments, including cold (4 C), drought (10% polyethylene glycol) and salt (200 mM NaCl), respectively

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Beijerinck found that when he tried to filter out the infectious agent, the cake (the material that was caught by the filter) would not pass on the disease, but the filtrate the solution that passes through the filter was infectious

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Then the laser is used to lighten the tissue to the desired color