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Acculturation stressors without stress-coping use A central finding was the explicit disconnection participants drew between their substantial adaptation challenges and e-cigarette use

Adolescents from lower-income households or deprived neighbourhoods often report higher rates of both smoking and vaping, raising concerns about the clustering of health-risk behaviours in disadvantaged settings [56, 57]

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The reverse transcription reaction was then carried out using High Capacity RNA to cDNA Kit (Invitrogen, USA) and followed by qPCR performed with specific TaqMan gene expression assays for FLG , LOR , IVL , KLK7 , TGM1 , and RPL13A genes (all ThermoFisher Scientific, USA) according to the manufacturers recommendations

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