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The measurement of the length of L1 and L3 larvae obtained from egg batches laid by e-nicotine- and water-exposed females was carried out from the most proximal to the most distal point of the body with an OLYMPUS SZX16 stereomicroscope using Olympus cell-Sens Standard imaging software (Olympus cellSens Standard, Version 1.16, Olympus Corporation, Tokyo, Japan)

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The plate was covered and incubated for one hour at room temperature without agitation

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