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Regarding the bacterial community at the phylum level, Firmicutes emerged as the most abundant taxon, constituting over 70% of the bacterial community (Figure 6A), which is consistent with previous results ( Staphylococcus was the most prevalent across all samples, with relative abundances of 81.39% in T0, 71.65% in T1, 74.29% in T2, and 72.31% in T3

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A 25 L reaction mixture was prepared, comprising 12.5 L PCR Master Mix (2X) (Thermo Scientific), 0.8 L of 10 mM forward primer, 0.8 L of 10 mM reverse primer, 2 L MT-DNA (20 ng/L), and 8.9 L nuclease-free water