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Alkaline phosphatase (ALP) activity The cell lysates were harvested in 1 Passive Lysis Buffer (Promega, Madison, WI) and clarified by centrifugation at 13,000 g for 20 min at 4C

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Integration of the FCTC in the SDG Agenda 2030 provides national planning opportunities for governments to demonstrate fulfilment of their treaty obligations to implement tobacco supply measuressuch as sales restrictions to minors, alternative livelihoods support, and illicit trade controlsas well as demand-reduction measures such as smoke-free policies, packaging and labeling provisions, marketing bans, cessation programs, and raising tobacco excises taxes

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