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A burn is typically sharply demarcated, sits in a location consistent with the reported insult (next to the offending tooth, on the palate after a hot meal, along the gum line for whitening), and matches the timeline

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Open the pack of cigarettes and take one by sliding your finger up

Therefore, to ensure that the increased toxicity of hP301L was not simply an effect of greater expression level compared to hTau, we assessed tau expression levels in all captured cells at 24 and 48 h, by measuring the intensity of V5 staining (Fig EV3D and E)