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Both plas- mid PGEX-6P-1 (Novagen) and CP were digested with BamH I (NEB, 10 units/L)/Xho I (NEB, 10 units/L) and cloned into the same sites in PGEX-6P-1 (PGEX- 6P-1-WT-GST-TMV-CP 32 )

Woodbines cigarettes became widespread during World War II

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3.3 Why It Beats Traditional Detection Methods Historically, hotels relied on: Housekeeper reports based on smell Physical evidence , like ash or cigarette butts Guest complaints from neighboring rooms These methods are subjective, inconsistent, and reactive