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The whole process is pretty straightforward: you use whats called the 'chew and park' method

Although amplicon pyrosequencing has major advantages for human microbiome studies, it has also some limitations, such as the possible overestimation of OTU richness due to homopolymer errors (repeated nucleotides), inaccuracies of taxonomic identification due to the short length of the sequences and the introduction of primer and sequencing related biases 50,51,52

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