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The resulting solution was injected (5 l) onto a 1200 series LC system (Agilent Technologies), consisting of 1200 series binary pump, autosampler, vacuum degasser, column compartment and control module, with a Luna 3 m C18(2) 100 , 100 mm 2.00 mm column and a SecurityGuard cartridge kit (Phenomenex, Macclesfield, UK) as a guard column

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11 Smoking driven by the need to satiate cravings cannot be described as a free choice