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Then, the membranes were incubated with primary antibodies against -actin (Abcam, MA, USA, ab189073), MMP-2 (Cell signaling #4022), MMP-9 (Cell Signaling #13,667), SERPINB2 (Abcam, MA, USA, ab47742), and caspase-3 (Cell signaling, MA, USA, #9662), overnight at 4 C and then with HRP-conjugated goat anti-rabbit IgG (BD Pharmingen, San Diego, CA, USA, 554,021) and goat anti-mouse IgG (BD Pharmingen, 554,002) secondary antibodies for 60 min at RT

doi:10.1089/ast.2010.0603